Selective Inhibition of IκBα Phosphorylation and HIV-1 LTR-Directed Gene Expression by Novel Antioxidant Compounds
نویسندگان
چکیده
منابع مشابه
Inhibition of HIV-LTR gene expression by oligonucleotides targeted to the TAR element.
All human immunodeficiency virus mRNAs contain a sequence known as TAR (trans-activating responsive sequence). The TAR element forms a stable RNA stem-loop structure which binds the HIV tat (trans-activator) protein and mediates increased viral gene expression. In principle, molecules which bind to the TAR RNA structure would inhibit trans-activation by perturbing the native RNA secondary struc...
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The treatment of HIV-1 infected patients is mostly based on compounds that directly target the activity of proteins coded by the virus. However, the viral genome mutates at a rapid rate, generating drug resistant strains, hence the need for novel drugs to outpace the adaptive mechanisms of the virus. Since cellular factors, which are required for the efficient expression of the HIV-1 genome, do...
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Many regions of the HIV-1 genome have been targeted in earlier studies by RNA-cleaving DNA enzymes possessing the 10-23 catalytic motif, and efficient inhibition of HIV-1 gene expression was reported. All these studies employed charged synthetic lipids to introduce the catalytic DNA into the mammalian cells, which severely limits its practical application and usefulness in vivo. Taking advantag...
متن کاملInhibition of HIV-1 replication by RNA targeted against the LTR region.
OBJECTIVE The use of small RNA molecules able to effect gene inactivation has emerged as a powerful method of gene therapy. These small inhibitory RNAs are widely used for silencing malignant cellular and viral genes. We have assayed a series of inhibitory RNAs named catalytic antisense RNAs, consisting of a catalytic domain, hairpin or hammerhead ribozyme, and an antisense domain. The aim of t...
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A library of three synthetic ribozymes with randomized arms, targeting NUX, GUX and NXG triplets, respectively, were used to identify ribozyme-accessible sites on the HIV-1 LTR transcript comprising positions -533 to 386. Three cleavable sites were identified at positions 109, 115 and 161. Ribozymes were designed against these sites, either unmodified or with 2'-modifications and phosphorothioa...
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ژورنال
عنوان ژورنال: Virology
سال: 1997
ISSN: 0042-6822
DOI: 10.1006/viro.1997.8642